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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="research-article" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">Russian Journal of Dentistry</journal-id><journal-title-group><journal-title xml:lang="en">Russian Journal of Dentistry</journal-title><trans-title-group xml:lang="ru"><trans-title>Российский стоматологический журнал</trans-title></trans-title-group></journal-title-group><issn publication-format="print">1728-2802</issn><issn publication-format="electronic">2413-2934</issn><publisher><publisher-name xml:lang="en">Eco-Vector</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">646209</article-id><article-id pub-id-type="doi">10.17816/dent646209</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>Experimental and Theoretical Investigations</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>Экспериментально-теоретические исследования</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title xml:lang="en">Periodontal tissue changes under chronic tobacco intoxication in an experimental model of gingival recession: a laser Doppler flowmetry study in rats</article-title><trans-title-group xml:lang="ru"><trans-title>Изменения в тканях пародонта при хронической табачной интоксикации на экспериментальной модели рецессии десны (по данным лазерной допплеровской флоуметрии): экспериментальное исследование на крысах</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7221-7976</contrib-id><contrib-id contrib-id-type="spin">5951-4659</contrib-id><name-alternatives><name xml:lang="en"><surname>Trunin</surname><given-names>Dmitry A.</given-names></name><name xml:lang="ru"><surname>Трунин</surname><given-names>Дмитрий Александрович</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>MD, Dr. Sci. (Medicine), Professor</p></bio><bio xml:lang="ru"><p>д-р мед. наук, профессор</p></bio><email>trunin-027933@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8232-5153</contrib-id><contrib-id contrib-id-type="spin">2173-4313</contrib-id><name-alternatives><name xml:lang="en"><surname>Kostrigina</surname><given-names>Ekaterina D.</given-names></name><name xml:lang="ru"><surname>Костригина</surname><given-names>Екатерина Дмитриевна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><email>KostriginaED1987@yandex.ru</email><xref ref-type="aff" rid="aff2"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9351-6177</contrib-id><contrib-id contrib-id-type="spin">1546-2237</contrib-id><name-alternatives><name xml:lang="en"><surname>Rubanenko</surname><given-names>Olesya A.</given-names></name><name xml:lang="ru"><surname>Рубаненко</surname><given-names>Олеся Анатольевна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>MD, Dr. Sci. (Medicine), Associate Professor</p></bio><bio xml:lang="ru"><p>д-р мед. наук, доцент</p></bio><email>dmib@samsmu.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0008-0683-2656</contrib-id><contrib-id contrib-id-type="spin">2575-4492</contrib-id><name-alternatives><name xml:lang="en"><surname>Zamyatin</surname><given-names>Yuri M.</given-names></name><name xml:lang="ru"><surname>Замятин</surname><given-names>Юрий Михайлович</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><email>yuran-best@yandex.ru</email><xref ref-type="aff" rid="aff2"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0002-0025-5276</contrib-id><name-alternatives><name xml:lang="en"><surname>Suntseva</surname><given-names>Maria M.</given-names></name><name xml:lang="ru"><surname>Сунцева</surname><given-names>Мария Михайловна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><email>mashakurbatova2346@gmail.com</email><xref ref-type="aff" rid="aff2"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0003-1983-5868</contrib-id><name-alternatives><name xml:lang="en"><surname>Eremeeva</surname><given-names>Anastasia D.</given-names></name><name xml:lang="ru"><surname>Еремеева</surname><given-names>Анастасия Дмитриевна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><email>anasteysha.eremeeva@bk.ru</email><xref ref-type="aff" rid="aff2"/></contrib></contrib-group><aff-alternatives id="aff1"><aff><institution xml:lang="en">Samara State Medical University</institution></aff><aff><institution xml:lang="ru">Самарский государственный медицинский университет</institution></aff></aff-alternatives><aff-alternatives id="aff2"><aff><institution xml:lang="en">Penza State University</institution></aff><aff><institution xml:lang="ru">Пензенский государственный университет</institution></aff></aff-alternatives><pub-date date-type="preprint" iso-8601-date="2025-03-07" publication-format="electronic"><day>07</day><month>03</month><year>2025</year></pub-date><pub-date date-type="pub" iso-8601-date="2025-04-29" publication-format="electronic"><day>29</day><month>04</month><year>2025</year></pub-date><volume>29</volume><issue>2</issue><issue-title xml:lang="en"/><issue-title xml:lang="ru"/><fpage>127</fpage><lpage>132</lpage><history><date date-type="received" iso-8601-date="2025-01-16"><day>16</day><month>01</month><year>2025</year></date><date date-type="accepted" iso-8601-date="2025-02-10"><day>10</day><month>02</month><year>2025</year></date></history><permissions><copyright-statement xml:lang="en">Copyright ©; 2025, Eco-Vector</copyright-statement><copyright-statement xml:lang="ru">Copyright ©; 2025, Эко-Вектор</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="en">Eco-Vector</copyright-holder><copyright-holder xml:lang="ru">Эко-Вектор</copyright-holder><ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/" start_date="2028-04-29"/><license><ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">https://creativecommons.org/licenses/by-nc-nd/4.0/</ali:license_ref></license></permissions><self-uri xlink:href="https://rjdentistry.com/1728-2802/article/view/646209">https://rjdentistry.com/1728-2802/article/view/646209</self-uri><abstract xml:lang="en"><p><bold>Background:</bold> At present, microcirculatory disturbances in organs and tissues have emerged as a critical issue in both experimental and clinical medicine, including periodontology. The findings presented in this article underscore the need for early detection of microcirculatory disorders in the periodontium under conditions of tobacco intoxication, using laser Doppler flowmetry. This diagnostic method, which records changes in microvascular blood flow by detecting signals from moving erythrocytes within vessels, provides a clear assessment of regional tissue perfusion.</p> <p><bold>Aim: </bold>To evaluate periodontal changes in rats with gingival recession under conditions of chronic tobacco intoxication (CTI), using laser Doppler flowmetry (LDF).</p> <p><bold>Methods:</bold> To experimentally validate the use of LDF for detecting periodontal tissue damage, microcirculation in the periodontium was assessed using the LDF analyzer LAKK-02, version 4 (NPP Lazma, Russia). The study included 75 adult male Wistar rats, which were divided into five groups: control; CTI; gingival recession; gingival recession elimination under continued CTI; and gingival recession elimination under CTI with a temporary cessation protocol.</p> <p><bold>Results:</bold> LDF revealed progression of pathological changes in periodontal tissues under conditions of tobacco intoxication and gingival recession, as evidenced by a decrease in connective tissue cell density and epithelial layer thickness.</p> <p><bold>Conclusion:</bold> Noninvasive LDF provides qualitative insights into the condition of the periodontal complex and enables early identification of tissue pathology, facilitating timely therapeutic intervention.</p></abstract><trans-abstract xml:lang="ru"><p><bold>Обоснование. </bold>В настоящее время нарушения микроциркуляторного русла органов и тканей выдвинулись в ряд важнейших проблем экспериментальной и клинической медицины, в том числе и пародонтологии. Представленные в статье данные указывают на необходимость раннего выявления признаков поражения микроциркуляции пародонта в условиях табачной интоксикации с помощью метода лазерной допплеровской флоуметрии. Этот метод диагностики, основанный на регистрации изменений в микроциркуляторном русле за счёт детектирования сигналов движущихся в сосудах эритроцитов, даёт чёткое представление об интенсивности кровоснабжения исследуемой области.</p> <p><bold>Цель.</bold> Оценить на крысах изменения пародонта при рецессии десны и в условиях хронической табачной интоксикации (ХТИ) с применением метода лазерной допплеровской флоуметрии (ЛДФ).</p> <p><bold>Материалы и методы.</bold> С целью экспериментального обоснования использования ЛДФ для выявления признаков поражения пародонта измеряли микроциркуляцию в тканях пародонта методом ЛДФ на лазерном анализаторе ЛАКК-02 в исполнении 4 (НПП «Лазма», Россия) у 75 половозрелых самцов крыс линии Wistar, разделённых на 5 групп: контрольную; в условиях ХТИ; в условиях рецессии десны; в условиях устранения рецессии десны с сохранением ХТИ; в условиях устранения рецессии десны с сохранением ХТИ с протоколом временной отмены.</p> <p><bold>Результаты.</bold> С использованием метода ЛДФ зарегистрировано течение патологического процесса в тканях пародонта в условиях табачной интоксикации и рецессии десны, поскольку было зафиксировано уменьшение количества клеток соединительной ткани и толщины эпителиального слоя.</p> <p><bold>Заключение.</bold> Неинвазивный метод ЛДФ даёт качественное представление о состоянии пародонтального комплекса, позволяет заранее определить патологическое состояние изучаемых тканей и начать своевременное лечение.</p></trans-abstract><kwd-group xml:lang="en"><kwd>laser doppler flowmetry</kwd><kwd>LDF</kwd><kwd>periodontitis diagnosis</kwd><kwd>tobacco intoxication</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>лазерная допплеровская флоуметрия</kwd><kwd>ЛДФ</kwd><kwd>диагностика пародонтита</kwd><kwd>табачная интоксикация</kwd></kwd-group><funding-group/></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>Zharkikh EV, Makovik IN, Potapova EV, et al. 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