Preservation solutions in traumatic dental injuries: A systematic review

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Abstract

BACKGROUND: Traumatic dental injuries caused by mechanical stimuli account for approximately 20% of dentofacial disorders. Dental replantation is currently viewed as a viable alternative to dental prosthetic rehabilitation and implantation. The outcome of replantation in traumatic dental injuries is largely determined by timely treatment and transportation conditions.

AIM: To perform literature search and review publications that assess and compare solutions for preserving the viability of teeth in traumatic dental injuries.

MATERIALS AND METHODS: The literature search was performed in PubMed and eLIBRARY.RU databases for the period between 1994 and 2023, in accordance with PRISMA 2020 guidelines. The review included publications that addressed the biological properties of solutions for preserving the viability of teeth in traumatic dental injuries.

RESULTS: The search yielded 252 articles. Following screening and full-text analysis, 36 articles comparing the efficacy of different transportation media were included in the review. The literature review identified 34 different solutions, with the efficacy assessed by viable periodontal cell counts. The analyzed solutions included Hank’s balanced salt solution, various types of milk, propolis, coconut water, and many others. The studies revealed considerable differences in the efficacy of analyzed solutions, storage conditions, and viable cell evaluation methods. The MTT assay and trypan blue were the most frequently used laboratory tests for assessing metabolic activity. According to the majority of authors, Hank’s balanced salt solution and milk were the best storage media for preserving the viability of tooth tissues.

CONCLUSION: The studies assessing solutions for preserving the viability of tooth cells in traumatic dental injuries highlight the need for more accessible and effective transportation media due to inconsistent data on their biological efficiency; further research is required.

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About the authors

Ekaterina A. Mitkina

Northern State Medical University

Author for correspondence.
Email: miekandr@yandex.ru
ORCID iD: 0000-0002-5631-5197
SPIN-code: 5996-4317
Russian Federation, Arkhangelsk

Ekaterina V. Strelnikova

Northern State Medical University

Email: katyusha.strelnikova.02@list.ru
ORCID iD: 0009-0000-9951-9547
SPIN-code: 8811-5405
Russian Federation, Arkhangelsk

Maria A. Gorbatova

Northern State Medical University

Email: marigora@mail.ru
ORCID iD: 0000-0002-6363-9595
SPIN-code: 7732-0755

MD, Cand. Sci. (Medicine), Associate Professor

Russian Federation, Arkhangelsk

Leonid L. Shagrov

Northern State Medical University

Email: leonidshagrov@mail.ru
ORCID iD: 0000-0003-2655-9649
SPIN-code: 3842-2145
Russian Federation, Arkhangelsk

Andrey M. Grzhibovsky

Northern State Medical University; M.K. Ammosov North-Eastern Federal University; M.V. Lomonosov Northern (Arctic) Federal University

Email: a.grjibovski@yandex.ru
ORCID iD: 0000-0002-5464-0498
SPIN-code: 5118-0081

MD, Dr. Sci. (Medicine), Professor

Russian Federation, Arkhangelsk; Yakutsk; Arkhangelsk

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2. Таблица 1. Растворы, которые использовались исследователями для оценки жизнеспособности поврежденных зубов.
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3. Fig. 1. Flowchart of the study selection process for inclusion in the review (according to PRISMA 2020 recommendations). © Eco-Vector, 2025.

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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
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