Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus

V. I. Timofeev; Тимофеев В. И.; Yu. A. Abramchik; Абрамчик Ю. А.; N. E. Zhukhlistova; Жухлистова Н. Е.; O. O. Mikheeva; Михеева О. О.; M. B. Shevtsov; Шевцов М. Б.; E. A. Zayats; Заяц Е. А.; D. D. Lykoshin; Лыкошин Д. Д.; M. A. Kostromina; Костромина М. А.; R. S. Esipov; Есипов Р. С.; I. P. Kuranova; Куранова И. П.

doi:10.31857/S0023476122060273

Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus

作者: Timofeev V.I.¹^,2, Abramchik Y.A.¹^,3, Zhukhlistova N.E.¹, Mikheeva O.O.³, Shevtsov M.B.⁴, Zayats E.A.³, Lykoshin D.D.³, Kostromina M.A.³, Esipov R.S.³, Kuranova I.P.¹^,2
隶属关系:
1. Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia
2. National Research Centre “Kurchatov Institute,”, 123098, Moscow, Russia
3. Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
4. Moscow Institute of Physics and Technology, 141701, Dolgoprudny, Moscow oblast, Russia
期: 卷 68, 编号 1 (2023)
页面: 46-50
栏目: STRUCTURE OF MACROMOLECULAR COMPOUNDS
URL: https://rjdentistry.com/0023-4761/article/view/673539
DOI: https://doi.org/10.31857/S0023476122060273
EDN: https://elibrary.ru/DMUZXL
ID: 673539

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详细

The high-producing strain С3029/pGro7/pERDerp3 for the house dust mite allergen Der p 3 from Dermatophagoides pteronyssinus, expressing the recombinant protein in Escherichia coli in the soluble form, was constructed. A procedure was developed for the purification of the recombinant allergen. Crystals of the recombinant protein Der p 3 suitable for X-ray diffraction analysis were grown by the vapor-diffusion method. The X-ray diffraction data set was collected to 2.25 Å resolution at the European Synchrotron Radiation Facility (ESRF, France, ID23-1 beamline) at 100 K. The crystals belong to sp. gr. С121 and contain two enzyme molecules per asymmetric unit.

关键词

STRUCTURE OF MACROMOLECULAR COMPOUNDS

作者简介

V. Timofeev

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; National Research Centre “Kurchatov Institute,”, 123098, Moscow, Russia

Email: inna@crys.ras.ru
Россия, Москва; Россия, Москва

Yu. Abramchik

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

Email: inna@crys.ras.ru
Россия, Москва; Россия, Москва

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