Restoration of normal blood coagulation with chitosan in the simulation of hypocoagulation with aspirin

The study of changes in blood clotting parameters under in vitro conditions with different incubation durations (5 min and 30 min) of chitosan with normal and with hypocoagulation plasma of rats was carried out. The addition of chitosan to the plasma of normal animals led to an increase in its procoagulant activity regardless of the duration of incubation. However, an increase in the incubation time to 30 min contributed to a more significant decrease in the fibrin-depolymerization activity of the blood. Incubation of chitosan with hypocoagulation plasma obtained after administration of acetylsalicylic acid (aspirin) to animals (once, orally at a dose of 2 mg / kg) for both 5 minutes and 30 minutes led to pronounced activation of blood clotting. Under these conditions, the lengthening of the incubation time caused more pronounced effects of this drug: a significant increase in the concentration of fibrinogen, ADP-dependent platelet aggregation, the degree of fibrin polymerization and a decrease in non-enzymatic fibrinolysis. At the same time, most of the studied hemostasis parameters approached values corresponding to the normal level. The results obtained in this work indicate the high effectiveness of chitosan as a means of restoring normal blood clotting, blocking the phenomena of bleeding in hypocoagulation conditions.