The characteristic of amphichiral nature of the distribution of periodontal bacteria in patients presenting with chronic generalized periodontitis

The species composition of type I pathogenic periodontal microflora was identified by the multiplex PCR technique. The study revealed the presence of Porphyromonas gingivalis, Prevotella intermedia, Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans), Tannerella forsythia (Bacteroides forsythus), and Treponema denticola. The samples were taken from 300 periodontal pockets localized in symmetric regions of the oral cavity of 75 patients presenting with chronic generalized periodontitis. It was shown that 1/5 of the patients had type I paradontopathogenic bacteria in the examined regions; they were absent in 1/4 of the patients. The pigment-producing bacterial species (P. intermedia and P. gingivalis) could be found in 3 or 4 quadrants of the dentogingival sulcus with a probability of 50-70% whereas T. denticola and A. actinomycetemcomitan occurred only in one quadrant in 47-56% of the cases. The data obtained can be used to estimate the role of local and systemic etiological factors in the susceptibility of the organism and their contribution to the development of various pathological conditions. Moreover, they are of importance for the clinical diagnostics and treatment of inflammatory diseases of the oral cavity.

periodontitis, pathogenic periodontal microflora, multiplex PCR