Determination of the minimum suppressing concentration to metronidazole in representatives of obligate and optional anaerobic microflora of periodontal pockets

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Abstract

BACKGROUND: Due to the long period of use of metronidazole in medical dental practice, today it is relevant to study the sensitivity and the minimum inhibitory concentration of reference and clinical strains of obligate anaerobic microbiota to metronidazole in periodontitis.

AIM: Determination of sensitivity and minimum suppressive concentration of reference and clinical strains of obligate anaerobic microbiota to metronidazole in periodontitis.

MATERIALS AND METHODS: Strains of obligate anaerobic microbiota of periodontal pockets with moderate periodonitis were isolated from 30 patients. The study was carried out by the cassette micromethod, which is a modification of the method for determining the sensitivity by serial dilution in a dense agar medium.

RESULTS: As a result of the study, it was found that many of the microbes found in periodontal pockets have a relatively low sensitivity to metronidazole (MIC from 4 to 12 μg / ml). This circumstance is obviously due to the fact that this antibacterial chemotherapy drug has been used for a long time in periodontal practice.

CONCLUSIONS: In this regard, in order to increase the effectiveness of local antibacterial therapy, this chemotherapy drug must be combined with antiseptics that have a pronounced antibacterial effect against periodontal pathogenic microflora, for example, Metrogyl Dent gel, containing, along with metronidazole, the antiseptic chlorhexidine, as well as the use of physical hardware methods of exposure to create significant the concentration of the antibacterial drug in the mucous membrane and in the contents of periodontal pockets, which can be done using phonophoresis.

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About the authors

Egor E. Olesov

Burnasyan Federal Medical Biophysical Center

Email: olesov_georgiy@mail.ru

MD, Dr. Sci. (Med.), Professor

Russian Federation, Moscow

Dmitry I. Morozov

Burnasyan Federal Medical Biophysical Center

Email: info@stomatologclub.ru
Russian Federation, Moscow

Alexander G. Volkov

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: parodont@inbox.ru
ORCID iD: 0000-0003-2674-1942

MD, Dr. Sci. (Med.), Professor

Russian Federation, 2, Bolshaya Pirogovskaya str., 119991, Moscow

Nataliya Zh. Dikopova

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Author for correspondence.
Email: zubnoy-doctor@yandex.ru
ORCID iD: 0000-0002-4031-2004

MD, Cand. Sci. (Med.), assistant professor

Russian Federation, 2, Bolshaya Pirogovskaya str., 119991, Moscow

Anna G. Ponomareva

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: zubnoy-doctor@yandex.ru
ORCID iD: 0000-0002-2685-7235

MD, Dr. Sci. (Med.), Professor

Russian Federation, 2, Bolshaya Pirogovskaya str., 119991, Moscow

References

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  2. Lashko IS, Tsarev VN, Olesov EE, et al. Comparative microbiological research of sensitivity of microflora of the oral cavity to the drugs krezacin denta and Metrogyl denta. Russian Journal of Dentistry. 2019;23(3–4):149–152. (In Russ). doi: 10.18821/1728-2802-2019-23-3-4-149-152
  3. Tsarev VN, Yagodina EA, Tsareva TV, Nikolaeva EN. The impact of the viral-bacterial consortium on occurrence and development of chronic periodontitis. Parodontologiya. 2020;25(2):84–89. (In Russ). doi: 10.33925/1683-3759-2020-25-2-84-88
  4. Krivchikova AS, Sadkova EE. Sravnitel'nyi analiz antimikrobnykh preparatov dlya lecheniya khronicheskogo generalizovannogo parodontita. Bulletin of Medical Internet Conferences. 2015;5(10):1215–1216. (In Russ).
  5. Akhmedbaeva SS, Volkov AG, Dikopova NZh, et al. The use of ultrasound and ozone therapy in the complex treatment of periodontitis. Russian Journal of Dentistry. 2020;24(2):74–78. (In Russ). doi: 10.17816/1728-2802-2020-24-2-74-78
  6. Yefanov OI, Tsaryov VN, Nosik AS, et al. In vitro study of the antibacterial activity of apex-phoresis using silver-copper electrode. Russian Journal of Dentistry. 2006;(4):1–6. (In Russ).
  7. Efanov OI, Tsarev VN, Volkov AG, et al. Otsenka antibakterial'noi aktivnosti apeks-foreza. Stomatology. 2006;85(5):20. (In Russ).

Supplementary files

Supplementary Files
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1. Fig. 1. Scheme for determining the sensitivity of microbial cultures to metronidazole in wells. * Control seeding (without adding to the well metronidazole microcassettes).

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2. Fig. 2. Colonies of Peptococcus niger on the wells of microcas-settes. View through a binocular magnifier.

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