Vol 22, No 5 (2018)

The purpose of the study-to justify the effectiveness of the method of Raman-fluorescence diagnostics in the assessment of the remineralisation of the hard tissues of the tooth. In preclinical in vitro study on the model 25 the test object teeth (the incisors of the lower jaw) removed as clinically indicated, carried out Raman-fluorescence spectroscopy with a wavelength of probe radiation of 532 nm. The advantages of Raman-fluorescence spectroscopy are expressiveness, noninvasiveness, simple and non-destructive control of the degree of remineralization of hard tooth tissues [1-3]. In the course of the study, a qualitative and quantitative analysis of the content and distribution of hydroxyapatite in the enamel in the normal and with the use of remineralizing drugs was carried out. The high sensitivity and reproducibility of the method allowed us to assess the degree of mineralization of the tooth, the effectiveness of remineralizing drugs.

Object: assessment of the antibacterial effect of sealing materials based on epoxy resin, zinc-eugenol of cement and hydroxide of calcium. Materials and methods: Antibacterial activity was estimated by agaro-diffusive test (ADT) estimated the of microorganisms. Plates from filling materials were placed on Petri dish, the sowed with St. aureus, and a zone of a growth inhibition of microorganisms was estimated. Materials were used right after mixing, right after hardening, in 30 days after mixing. Results: Materials had the maximum antibacterial effect after hardening, minimum in 30 days after mixing. The greatest efficiency against St. aureus showed material based on zinc-oxide- eugenol cement.

Justification. Information on objective data and reference indicators characterizing the state and relationship of the maxillofacial organs with the General adaptive syndrome and stress is poorly understood. The purpose of research: experimental and clinical justification of the method of complex and rapid diagnosis of stress as a manifestation of AOS in students on metabolic, morphometric and functional parameters of tissues of the maxillofacial region. Materials and methods. The study involved 30 students aged 18 to 21 years (17 females and 13 males) in the period before and after training sessions with mental stress, after physical exertion, as well as after the end of the first year of study, at the beginning and in the middle of the first semester of the second year of study. The following tests were performed: electrodontodiagnosis, pH-metry and fluorescence diagnostics of saliva, thermometry of periodontal tissues, Rod test, calculation of the Sheikh-Zade formula, spielberger psychological test. Statistical analysis of the results was carried out using standard statistical complexes in Microsoft Excel. Results. EDI: after two sessions with mental stress - an increase of 46.5% (p<0.01). After physical unloading - recovery by 23.2% (p<0.05). pH-metry of saliva: at the end of the school year of 6.41 ± 0,19, after two months of summer vacation - of 6.87± 0,11, and in the middle of the first semester of the second year - of 6.63 ± 0,11. Comparative analysis of thermometry parameters of periodontal tissues in students at the end of the school year and after two months of rest - an increase in gum temperature, on average, by 0.62 °C (p<0.05). When measuring the fluorescence intensity of saliva samples before and after the training load in comparison with the initial state, it was revealed a significant increase in the wavelength range of 600-650 nm and an increase in the integral intensity of 10-30 times, and after a night rest - a decrease of 7-10 times. Test bar: after two sessions with mental stress - deterioration by 31.5% (p<0.001), after physical unloading - improvement by 19.3% (p<0.001). Formula Sheikh Zade: after two sessions with mental stress-an increase of 57.2% (p<0.001), after physical unloading - the dynamics of a decrease of 18.6% (p<0.05). Psychological test of spielberger after summer vacation: prevalence of average level of personal anxiety and high level of reactive anxiety. Conclusion: these methods of examination can be reasonably included in the method of complex diagnosis of CCA and its correction in a wide range of students at all stages of the educational process. The presented indicators of the maxillofacial area objectively and multifactorially reflect the dental status of the CHLO and can be used as a reference in the assessment of stress loads in students at the stages of their training.

The article presents the results of microbiological study of the content of periodontal pockets of patients with rheumatoid arthritis in combination with chronic periodontitis of varying severity.

Introduction: β-thalassemia major is a hereditary blood disease, a main pathogenetic factor of which is the disruption of the synthesis of β-chains of hemoglobin. Inevitable metabolic disorders that occur during regular blood transfusion form an additional risk of damage and cellular modification of organs and tissues, including the oral cavity. Aim: determination of the pathogenetic role of iron metabolism disorder in the development of chronic inflammatory periodontal diseases in patients with β-thalassemia. Material and methods: 12 patients with β - thalassemia major who had periodontal diseases were examined in the Republican Center of Thalassemia in Baku (average age is 18 ± 1.3 years). The control group consisted of 16 somatically healthy individuals with intact gingiva tissues (the average age is 18.0 ± 0.7 years). Serum Iron, ferritin, hepcidin, total iron-binding capacity (TIBC), level of Il2, Il6, Il10, TNFα were measured from peripheral blood samples. To determine the ultra-structural study of the periodontal tissues in patients with β-thalassemia major, the method of electron microscopy studies of gingiva biopsies was used. Results of the study: The study revealed an increase in the level of serum iron and ferritin against the background of a progressively decreasing TIBC in patients with β-thalassemia major. Also activation of pro-inflammatory cytokines - TNFα, Il6, Il10 was observed in homozygous patients. Metabolic indicators are compared with ultrastructural features of cellular elements of a gum. Signs of accumulation of iron in cellular elements both of its own plate and of the epithelial cover were compared. The presence of iron was recorded in immunocompetent cells - macrophages and lymphocytes. Conclusion: Summarizing the data obtained, we can conclude that deproteinization of ferritin cores and their detection both in the cytoplasm and in the nucleoplasm of immunocompotent cells leads to irreversible degenerative changes in cellular elements of the gingiva. The authors link the development of the generalized inflammatory - degenerative process in the periodontium with revealed exchange -structural violations.

The analysis of 80 clinical observations in which sialo-endoscopy was used as a diagnosis of the cause of obstruction of the salivary gland was performed. The study showed that endoscopy of the ducts of the major salivary glands provides important diagnostic information inaccessible to other methods. The information obtained with endosialoscopy is necessary for the correct choice of treatment for patients with obstructive sialoadenitis.